Document Type
Article
Publication Title
Journal of Cell Science
Abstract
Secretory cargo is recognized, concentrated and trafficked from endoplasmic reticulum (ER) exit sites (ERES) to the Golgi. Cargo export from the ER begins when a series of highly conserved COPII coat proteins accumulate at the ER and regulate the formation of cargo-loaded COPII vesicles. In animal cells, capturing live de novo cargo trafficking past this point is challenging; it has been difficult to discriminate whether cargo is trafficked to the Golgi in a COPII-coated vesicle. Here, we describe a recently developed live-cell cargo export system that can be synchronously released from ERES to illustrate de novo trafficking in animal cells. We found that components of the COPII coat remain associated with the ERES while cargo is extruded into COPII-uncoated, non-ER associated, Rab1 (herein referring to Rab1a or Rab1b)-dependent carriers. Our data suggest that, in animal cells, COPII coat components remain stably associated with the ER at exit sites to generate a specialized compartment, but once cargo is sorted and organized, Rab1 labels these export carriers and facilitates efficient forward trafficking.
DOI
10.1242/jcs.239814
Publication Date
7-1-2020
Recommended Citation
Westrate, Laura M.; Hoyer, Melissa J.; Nash, Michael J.; and Voeltz, Gia K., "Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport" (2020). University Faculty Publications and Creative Works. 187.
https://digitalcommons.calvin.edu/calvin_facultypubs/187