Document Type
Article
Publication Title
Journal of Physical Chemistry B
Abstract
Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA2.7, G5-FA4.7, and G5-FA7.2, respectively. Folic acid blocking experiments for G5-FA7.2reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone. (Graph presented).
First Page
11506
Last Page
11512
DOI
10.1021/acs.jpcb.5b05391
Publication Date
9-3-2015
Recommended Citation
Leroueil, Pascale R.; Dimaggio, Stassi; Leistra, Abigail N.; and Blanchette, Craig D., "Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study" (2015). University Faculty Publications and Creative Works. 236.
https://digitalcommons.calvin.edu/calvin_facultypubs/236