Saccharedens versatilis gen. Nov., sp. nov., a sugar-degrading member of the burkholderiales isolated from Cephalotes rohweri ant guts

Authors

Jonathan Y. Lin, Calvin UniversityFollow
William J. Hobson, Calvin UniversityFollow
John T. Wertz, Calvin UniversityFollow

Document Type

Article

Publication Title

International Journal of Systematic and Evolutionary Microbiology

Abstract

Cephalotes ‘turtle’ ants host a core group of gut–associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized in vitro. To gain a better understanding of the metabolic capability of core symbionts belonging to the Burkholderiales, we cultivated and characterized strain CAG32T from the guts of Cephalotes rohweri ants. Strain CAG32T was rod-shaped, Gram-stain-negative, motile and formed pale-white colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 20% O2 supplemented with 1% CO2. Strain CAG32T grew under NaCl concentrations of 0–2.0%, temperatures of 23–47 °C and pH values of 4.0–8.0, and was capable of producing n-butyric acid and degrading carbohydrates for growth. The G+C content of the genomic DNA was 59.2±0.6 mol% and the major fatty acids were C16 :0, C16 :1ω7c/C16 :1ω6c, C17 :0 cylcopropane, C12 :0 and C14 :0 3-OH/C16 :1 iso I. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on phylogenetic analysis of the 16S rRNA gene sequence, strain CAG32T shared 96.9% nucleotide similarity with its closest cultivated neighbours Bordetella petrii Se-1111RT and Bordetella bronchiseptica ATCC 19395T. This, combined with differences in the phenotypic and biochemical profile from neighbouring strains, warrants the classification of strain CAG32T as representing a novel species of a new genus within the Burkholderiales family Alcaligenaceae. The name Saccharedens versatilis gen. nov., sp. nov. is proposed. The type strain of Saccharedens versatilis is CAG32T (=NCIMB 15010T=DSM 100909T).

First Page

447

Last Page

453

DOI

10.1099/ijsem.0.001650

Publication Date

2-1-2017

Recommended Citation

Lin, Jonathan Y.; Hobson, William J.; and Wertz, John T., "Saccharedens versatilis gen. Nov., sp. nov., a sugar-degrading member of the burkholderiales isolated from Cephalotes rohweri ant guts" (2017). University Faculty Publications and Creative Works. 238.
https://digitalcommons.calvin.edu/calvin_facultypubs/238