X-ray diffraction structures of some phosphatidylethanolamine lamellar and inverted hexagonal phases
Document Type
Article
Publication Title
Biophysical Journal
Abstract
X-ray diffraction is used to solve the low-resolution structures of fully hydrated aqueous dispersions of seven different diacyl phosphatidylethanolamines (PEs) whose hydrocarbon chains have the same effective chain length but whose structures vary widely. Both the lower-temperature, liquid-crystalline lamellar (Lα) and the higher-temperature, inverted hexagonal (H11) phase structures are solved, and the resultant internal dimensions (d-spacing, water layer thickness, average lipid length, and headgroup area at the lipid-water interface) of each phase are determined as a function of temperature. The magnitude of the Lα and H11 phase d-spacings on either side of the Lα/H11 phase transition temperature (Tη) depends significantly on the structure of the PE hydrocarbon chains. The Lα phase d-spacings range from 51.2 to 56.4 Å, whereas those of the H11 phase range from 74.9 to 82.7 Å. These new results differ from our earlier measurements of these PEs (Lewis et al., Biochemistry, 28:541-548, 1989), which found near constant d-spacings of 52.5 and 77.0-78.0 Å for the Lα and H11 phases, respectively. In both phases, the d-spacings decrease with increasing temperature independent of chain structure, but, in both phases, the rate of decrease in the Lα phase is smaller than that in the H11 phase. A detailed molecular description of the Lα/H11 phase transition in these PEs is also presented.
First Page
2693
Last Page
2706
DOI
10.1016/S0006-3495(01)75912-7
Publication Date
1-1-2001
Recommended Citation
Harper, Paul E.; Mannock, David A.; Lewis, Ruthven; and McElhaney, Ronald N., "X-ray diffraction structures of some phosphatidylethanolamine lamellar and inverted hexagonal phases" (2001). University Faculty Publications and Creative Works. 495.
https://digitalcommons.calvin.edu/calvin_facultypubs/495