Calvin Digital Commons - Summer Research: A Tale of Two Toxicants: How Trichloroethylene (TCE) and Perchloroethylene (PCE) Metabolites Affect Immune Cell Viability Upon Co-exposure with Lipopolysaccharide (LPS)
 

Start Date

2022

Description

  • TCE and PCE are volatile organic solvents used in industrial processes, such as metal degreasing and dry cleaning, and known human carcinogens.
  • GBS is a common bacteria in the vaginal flora but can lead to complications such as preterm birth during pregnancy.
  • Lipopolysaccharide (LPS) is a gram-negative bacterial cell wall component that induces inflammatory immune responses and is used as an infection model.
  • Co-treatment (GBS+DCVC) in placental membranes lead to inhibition of pathogen stimulated cytokine secretion (Fig. 1).
  • We hypothesized that DCVC and TCVC (PCE’s metabolite) would produce decreased cell viability, and DCVC+LPS would produce further immune inhibitory effects, which were measured using multiple cell viability kits.

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Jan 1st, 12:00 AM

A Tale of Two Toxicants: How Trichloroethylene (TCE) and Perchloroethylene (PCE) Metabolites Affect Immune Cell Viability Upon Co-exposure with Lipopolysaccharide (LPS)

  • TCE and PCE are volatile organic solvents used in industrial processes, such as metal degreasing and dry cleaning, and known human carcinogens.
  • GBS is a common bacteria in the vaginal flora but can lead to complications such as preterm birth during pregnancy.
  • Lipopolysaccharide (LPS) is a gram-negative bacterial cell wall component that induces inflammatory immune responses and is used as an infection model.
  • Co-treatment (GBS+DCVC) in placental membranes lead to inhibition of pathogen stimulated cytokine secretion (Fig. 1).
  • We hypothesized that DCVC and TCVC (PCE’s metabolite) would produce decreased cell viability, and DCVC+LPS would produce further immune inhibitory effects, which were measured using multiple cell viability kits.