Start Date
2022
Description
- We present a laboratory experiment in biochemistry to study the Far-UV Visible secondary structure of a protein (horseradish peroxidase, HRP) that can be adapted for a single-session laboratory experiment or a multiple-session laboratory project.
- A Circular Dichroism (CD) spectrometer provides structural information of proteins as a function of environmental changes such as pH and temperature. We present secondary structural information for the protein, horseradish peroxidase, at pH 3 and pH 7 in the temperature range 30-80° C in phosphate buffered solution (PBS).
- HRP has many applications in biochemistry and biotechnology. It is frequently used as a reporter enzyme in diagnostics and histochemistry (ELISA and Western Blot) and has found recent applications in targeted cancer therapies
Recommended Citation
Kamran, Shemaiah; E. Bultje, Sarah; and Sinniah, Kumar, "Using Circular Dichroism Spectroscopy to Study Secondary Structure of Horseradish Peroxidase: A Laboratory Experiment in Biochemistry" (2022). Summer Research. 5.
https://digitalcommons.calvin.edu/summer_research/2022/Posters/5
Included in
Jan 1st, 12:00 AM
Using Circular Dichroism Spectroscopy to Study Secondary Structure of Horseradish Peroxidase: A Laboratory Experiment in Biochemistry
- We present a laboratory experiment in biochemistry to study the Far-UV Visible secondary structure of a protein (horseradish peroxidase, HRP) that can be adapted for a single-session laboratory experiment or a multiple-session laboratory project.
- A Circular Dichroism (CD) spectrometer provides structural information of proteins as a function of environmental changes such as pH and temperature. We present secondary structural information for the protein, horseradish peroxidase, at pH 3 and pH 7 in the temperature range 30-80° C in phosphate buffered solution (PBS).
- HRP has many applications in biochemistry and biotechnology. It is frequently used as a reporter enzyme in diagnostics and histochemistry (ELISA and Western Blot) and has found recent applications in targeted cancer therapies