Start Date

2022

Description

  • We present a laboratory experiment in biochemistry to study the Far-UV Visible secondary structure of a protein (horseradish peroxidase, HRP) that can be adapted for a single-session laboratory experiment or a multiple-session laboratory project.
  • A Circular Dichroism (CD) spectrometer provides structural information of proteins as a function of environmental changes such as pH and temperature. We present secondary structural information for the protein, horseradish peroxidase, at pH 3 and pH 7 in the temperature range 30-80° C in phosphate buffered solution (PBS).
  • HRP has many applications in biochemistry and biotechnology. It is frequently used as a reporter enzyme in diagnostics and histochemistry (ELISA and Western Blot) and has found recent applications in targeted cancer therapies

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Jan 1st, 12:00 AM

Using Circular Dichroism Spectroscopy to Study Secondary Structure of Horseradish Peroxidase: A Laboratory Experiment in Biochemistry

  • We present a laboratory experiment in biochemistry to study the Far-UV Visible secondary structure of a protein (horseradish peroxidase, HRP) that can be adapted for a single-session laboratory experiment or a multiple-session laboratory project.
  • A Circular Dichroism (CD) spectrometer provides structural information of proteins as a function of environmental changes such as pH and temperature. We present secondary structural information for the protein, horseradish peroxidase, at pH 3 and pH 7 in the temperature range 30-80° C in phosphate buffered solution (PBS).
  • HRP has many applications in biochemistry and biotechnology. It is frequently used as a reporter enzyme in diagnostics and histochemistry (ELISA and Western Blot) and has found recent applications in targeted cancer therapies