Start Date
2023
Description
Type 1 Diabetes (T1D) is a disease characterized by the inability to produce insulin. Susceptibility to T1D is complex, involving both genetic and environmental factors. o One of the genetic components involved in insulin gene expression, and suspected T1D susceptibility, is the insulin-linked polymorphic region (ILPR). The ILPR element is located on chromosome 11p15 in the proximal promoter region, 363 base pairs upstream from the gene transcription site. o Other researchers (using fluorescence spectroscopy) and we (using ITC) have demonstrated that insulin binds to G-Quadruplex DNA with µM affinity. Since the DNA sequences in the ILPR form G-Quadruplexes and are in the promotor region, the hormone may play a role in regulating the expression of its own gene.
Recommended Citation
Bultje, Sarah E.; Witvliet, Luke D.; and Sinniah, Kumar, "Investigating the Binding of G-Quadruplex DNA to Lispro and Bovine Insulin" (2023). Summer Research. 5.
https://digitalcommons.calvin.edu/summer_research/2023/Posters/5
Included in
Investigating the Binding of G-Quadruplex DNA to Lispro and Bovine Insulin
Type 1 Diabetes (T1D) is a disease characterized by the inability to produce insulin. Susceptibility to T1D is complex, involving both genetic and environmental factors. o One of the genetic components involved in insulin gene expression, and suspected T1D susceptibility, is the insulin-linked polymorphic region (ILPR). The ILPR element is located on chromosome 11p15 in the proximal promoter region, 363 base pairs upstream from the gene transcription site. o Other researchers (using fluorescence spectroscopy) and we (using ITC) have demonstrated that insulin binds to G-Quadruplex DNA with µM affinity. Since the DNA sequences in the ILPR form G-Quadruplexes and are in the promotor region, the hormone may play a role in regulating the expression of its own gene.